TB-500 Reconstitution Calculator
Enter your TB-500 vial size, the bacteriostatic water you added, and an amount to measure. Get the exact insulin-syringe units that volume represents — live on the syringe below. It’s the same measurement math built into the PeptideWiz app.
Research compound — no established dosing. TB-500 is a research compound; it is not FDA approved and has no established human dosing. This page performs measurement arithmetic only — dosing decisions belong to a licensed provider. Every figure shown is an example calculation, not a recommended dose.
Common vial sizes
To measure an amount of 1 mg, pull the plunger to 40.0 units.
Not medical advice. This calculator performs arithmetic on the numbers you enter. TB-500 is a research compound with no FDA approval and no established human dosing; every figure here is an example calculation, not a recommended dose. It does not confirm that TB-500 or any protocol is safe or appropriate for you, or replace your provider. Always verify everything with a licensed medical professional.
Example mixing math (arithmetic only)
The water volume you add sets the concentration — it never changes the amount itself, only how big the draw is on the syringe. Here’s how a 5 mg vial reads on a U-100 insulin syringe at three water volumes. These are formula demonstrations, not suggested doses:
| Water added | Concentration | Example 1 mg draw |
|---|---|---|
| 1 mL | 5,000 mcg/mL | 20 units |
| 2 mL | 2,500 mcg/mL | 40 units |
| 3 mL | 1,667 mcg/mL | 60 units |
These are formula demonstrations, not suggested doses. Using a 10 mg vial? Tap the preset above — the calculator redoes every number instantly.
About TB-500 (research status)
TB-500 is a synthetic fragment of thymosin beta-4, a naturally occurring protein involved in cell migration and actin regulation. It has been studied mainly in preclinical and animal research relating to tissue repair, with only limited human clinical evidence. It is not FDA approved and has no established human dosing, and it is sold as a research chemical rather than a medicine.
Because TB-500 is handled at the milligram scale, example draws represent larger volumes than micrograms-scale peptides, so the water volume you choose matters for syringe fit — too little water can push a single draw past the capacity of a small insulin syringe. The calculator above lets you compare volumes; every number it returns is an example calculation only.
How to reconstitute a TB-500 vial
- Gather supplies. The lyophilized TB-500 vial, bacteriostatic water, a sterile mixing syringe, alcohol wipes, and your insulin syringes.
- Sanitize. Wash your hands and wipe both vial stoppers with a fresh alcohol wipe.
- Draw the water. Pull your chosen volume (for example 2 mL) into the mixing syringe — that volume sets the concentration, so enter the same number in the calculator above.
- Add it slowly. Let the water run gently down the inside wall of the vial. Don’t jet it straight onto the powder.
- Swirl, don’t shake. Gently swirl until the solution is completely clear — shaking can degrade peptides.
- Label and refrigerate. Write the date and concentration on the vial, store it cold, and track remaining volume and the reconstitution date in the PeptideWiz app.
Full walkthrough: How to reconstitute peptides, step by step →
How the calculation works
vial (mcg) ÷ water (mL)
How much TB-500 is in every millilitre after reconstitution.
amount (mcg) ÷ concentration
The millilitres that contain exactly the amount you entered.
mL × 100
A U-100 insulin syringe holds 100 units per millilitre.
Worked example: a 5 mg vial (5,000 mcg) with 2 mL of water gives 2,500 mcg/mL. A 1 mg (1,000 mcg) amount is 1,000 ÷ 2,500 = 0.4 mL, and 0.4 × 100 = 40 units — an example calculation, not a recommended dose.
Frequently asked questions
How much bacteriostatic water should I add to a 5 mg TB-500 vial?
The water only sets the concentration, not the amount. 2 mL gives 2,500 mcg/mL, so a 1 mg example draw is 40 units. Because TB-500 is measured in milligrams, larger water volumes keep a single draw within a syringe’s capacity; the calculator lets you compare instantly. TB-500 is a research compound with no established human dosing.
How many units is 1 mg of TB-500?
It depends entirely on your vial’s concentration. At 2,500 mcg/mL (5 mg vial + 2 mL water) it’s 40 units; at 5,000 mcg/mL (same vial + 1 mL) it’s 20 units. This is an example calculation only — calculate from your own vial and water volume.
Is TB-500 FDA approved, and does it have a recommended dose?
No. TB-500 is a synthetic fragment of thymosin beta-4 sold as a research chemical. It is not FDA approved, has been studied mainly in preclinical and animal research, and has no established human dose. This page does not recommend any dose — it performs measurement arithmetic on the numbers you enter.
Can PeptideWiz keep track of my research vials?
Yes — the PeptideWiz app logs reconstitution dates, tracks each measured draw, and keeps a running vial inventory with expiration alerts, so you always know a vial’s concentration and remaining volume.
Track your research vials in the PeptideWiz app →
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